Tunel Stain Method (TTM) is a stain that uses the presence or absence of certain proteins on the surface of cells to identify them. These proteins are called “tunnels” because they allow water molecules to pass through them. Tunnels are found in many types of tissues including blood vessels, nerves, cartilage, bone and skin. They may also occur in other cell types such as white blood cells, platelets and neutrophils.
The tunnelled pattern of a cell is often used to distinguish it from another type of cell. For example, if you were trying to determine whether a particular tissue contained cancerous cells, you would use the tunnelled pattern of a tumor. A tumor contains numerous small blood vessels which can only be seen under the microscope when viewed under high magnification.
By using tunnelling microscopy, you could see these tiny blood vessels while examining a tumor.
In addition to being used to differentiate one type of cell from another, tunnelling can also be used to diagnose disease. For example, if a patient develops a fever after receiving chemotherapy treatment, the doctor might suspect leukemia since there are several lymph nodes in the neck where leukemia usually occurs.
- In Situ Detection of Apoptosis by the TUNEL Assay: An Overview of Techniques (DT Loo – DNA Damage Detection In Situ, Ex Vivo, and In Vivo, 2011 – Springer)
- An overview of apoptosis assays detecting DNA fragmentation (P Majtnerová, T Roušar – Molecular biology reports, 2018 – Springer)
- An overview of the toxic effect of potential human carcinogen Microcystin-LR on testis (Y Lone, RK Koiri, M Bhide – Toxicology Reports, 2015 – Elsevier)